chip-based surface plasmon resonance (spr) biosensors Search Results


90
MagArray Inc magnetoresistive biosensor chips
Magnetoresistive Biosensor Chips, supplied by MagArray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore sensor chip cm5
Sensor Chip Cm5, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore c biosensor
C Biosensor, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore spr-based chip biacore systems
Spr Based Chip Biacore Systems, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore chip-based surface plasmon resonance (spr) biosensors
Chip Based Surface Plasmon Resonance (Spr) Biosensors, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore spr-based chip
Spr Based Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore sensor chip
Sensor Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biacore surface plasmon resonance biosensor
Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor <t>(SPR)</t> assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via <t>the</t> <t>BIAcore</t> T100 analysis software (BIAevaluation Version 3.1).
Surface Plasmon Resonance Biosensor, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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European Comission Reference Material rea based biosensor chip
Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor <t>(SPR)</t> assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via <t>the</t> <t>BIAcore</t> T100 analysis software (BIAevaluation Version 3.1).
Rea Based Biosensor Chip, supplied by European Comission Reference Material, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SI Manufacturing ge-based biosensors
Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor <t>(SPR)</t> assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via <t>the</t> <t>BIAcore</t> T100 analysis software (BIAevaluation Version 3.1).
Ge Based Biosensors, supplied by SI Manufacturing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABTECH Scientific iam chips
Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor <t>(SPR)</t> assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via <t>the</t> <t>BIAcore</t> T100 analysis software (BIAevaluation Version 3.1).
Iam Chips, supplied by ABTECH Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor (SPR) assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via the BIAcore T100 analysis software (BIAevaluation Version 3.1).

Journal: Viruses

Article Title: Repurposing of Doramectin as a New Anti-Zika Virus Agent

doi: 10.3390/v15051068

Figure Lengend Snippet: Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor (SPR) assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via the BIAcore T100 analysis software (BIAevaluation Version 3.1).

Article Snippet: Subsequently, the RdRp protein was immobilized on a CM5 sensor chip for the BIAcore assay based on the surface plasmon resonance biosensor (SPR) to investigate the direct interaction between doramectin and RdRp.

Techniques: Binding Assay, Generated, Solvent, Staining, SDS Page, Western Blot, Purification, SPR Assay, Software