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Image Search Results
Journal: Viruses
Article Title: Repurposing of Doramectin as a New Anti-Zika Virus Agent
doi: 10.3390/v15051068
Figure Lengend Snippet: Doramectin directly binds to ZIKV RdRp. ( A ) Structural model for doramectin binding to the ZIKV NS5 protein was performed with Molecular Operating Environment (MOE). The NS5 protein is displayed as ribbons with the RdRp domain in red, MTase domain in yellow, and doramectin shown as green sticks. ( B ) Two-dimensional ligand-interaction maps of co-crystals of ZIKV NS5 domains bound with doramectin were generated using MOE. Polar residues are colored light purple, charged residues have an additional blue ring, and lipophilic residues are green. The degree of solvent exposure is shown by the blue halos. H-bond interactions to the amino acid side chain or main chain are shown as dashed green arrows, pointing toward the H-bond acceptor. ( C ) Coomassie blue-stained SDS-PAGE (lane 1) of crude extracts and western blot probed with anti-ZIKV NS5 (lane 2) after purification. ( D , E ) The surface plasmon resonance biosensor (SPR) assay to examine and characterize the binding of doramectin ( D ) or ribavirinc ( E ) to ZIKV RdRp. The K d values were calculated via the BIAcore T100 analysis software (BIAevaluation Version 3.1).
Article Snippet: Subsequently, the RdRp protein was immobilized on a CM5 sensor chip for the
Techniques: Binding Assay, Generated, Solvent, Staining, SDS Page, Western Blot, Purification, SPR Assay, Software